PC Knockout cell line (THP-1)

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location： Home > Products > KO Cell Lines > PC Knockout cell line (THP-1)

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PC Knockout cell line (THP-1) Order

PC Knockout cell line (THP-1)
Catalog#
YKO-HT0715
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PC Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PC Knockout cell line (THP-1)
Species
Human
Gene Name
PC
Gene ID
5091
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript PC-203)
Validation: PCR+Sanger Sequencing
PC Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PC gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PC gene knockout PC Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PC Knockout cell line (THP-1)
PC Knockout cell line (THP-1)
Catalog#
YKO-HT0715
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PC Knockout cell line (A549)

    PC Knockout cell line (A549)
      inquiry   More details

    Organism:
    Gene Name: PC
    Gene ID: 5091
    Size: 1*10^6
    Catalog#: YKO-H1474
    YKO-H1474(A549)
  • PC Knockout cell line(HEK293)

    PC Knockout cell line(HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: PC
    Gene ID: 5091
    Size: 1*10^6
    Catalog#: YKO-H531
    YKO-H531(HEK293)
  • PC Knockout cell line (HeLa)

    PC Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: PC
    Gene ID: 5091
    Size: 1*10^6
    Catalog#: YKO-HS09236
    YKO-HS09236(HeLa)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

PC Knockout cell line (THP-1) Order

PC Knockout cell line (THP-1)
Catalog#
YKO-HT0715
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PC Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PC Knockout cell line (THP-1)
Species
Human
Gene Name
PC
Gene ID
5091
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript PC-203)
Validation: PCR+Sanger Sequencing
PC Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PC gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PC gene knockout PC Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PC Knockout cell line (THP-1)
PC Knockout cell line (THP-1)
Catalog#
YKO-HT0715
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PC Knockout cell line (A549)

    PC Knockout cell line (A549)
      inquiry   More details

    Organism:
    Gene Name: PC
    Gene ID: 5091
    Size: 1*10^6
    Catalog#: YKO-H1474
    YKO-H1474(A549)
  • PC Knockout cell line(HEK293)

    PC Knockout cell line(HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: PC
    Gene ID: 5091
    Size: 1*10^6
    Catalog#: YKO-H531
    YKO-H531(HEK293)
  • PC Knockout cell line (HeLa)

    PC Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: PC
    Gene ID: 5091
    Size: 1*10^6
    Catalog#: YKO-HS09236
    YKO-HS09236(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines


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